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. 2015 Oct 21;310(2):C99–C114. doi: 10.1152/ajpcell.00092.2015

Fig. 8.

Fig. 8.

Effect of ATP on Bcl-2 and Bax expression and Bcl-2/Bax ratio in normal and lung cancer cells after application of BAPTA-AM. A: representative traces showing changes in [Ca2+]cyt before, during, and after application of 100 μM ATP after treatment with 25 μM BAPTA-AM in normal and lung cancer cells. B: representative traces showing changes in [Ca2+]cyt before, during, and after application of 1 mM ATP after treatment with 25 μM BAPTA-AM in normal and lung cancer cells. C: Western blot analysis of Bcl-2 and Bax expression in normal and lung cancer cells treated with 100 μM ATP for 0, 2, 4, 8, 16, and 24 h after application of 25 μM BAPTA-AM. β-Actin was used as a control. D: Western blot analysis of Bcl-2 and Bax expression in normal and lung cancer cells treated with 1 mM ATP for 0, 2, 4, 8, 16, and 24 h after application of 25 μM BAPTA-AM. β-Actin was used as a control. E: summarized data (means ± SE) showing the changes of Bcl-2 and Bax expression level in normal and lung cancer cells treated with 100 μM ATP for 0, 2, 4, 8, 16, and 24 h after application of 25 μM BAPTA-AM. F: summarized data (means ± SE) showing the changes of Bcl-2/Bax ratio in normal and lung cancer cells treated with 100 μM ATP for 0, 2, 4, 8, 16, and 24 h after application of 25 μM BAPTA-AM (n = 5). G: summarized data (means ± SE) showing the changes of Bcl-2 and Bax expression level in normal and lung cancer cells treated with 1 mM ATP for 0, 2, 4, 8, 16, and 24 h after application of 25 μM BAPTA-AM. H: summarized data (means ± SE) showing the changes of Bcl-2/Bax ratio in normal and lung cancer cells treated with 1 mM ATP for 0, 2, 4, 8, 16, and 24 h after application of 25 μM BAPTA-AM (n = 5).