Skip to main content
. 2015 Oct 21;310(2):C99–C114. doi: 10.1152/ajpcell.00092.2015

Fig. 9.

Fig. 9.

Effect of ATP on Bcl-2 and Bax expression and Bcl-2/Bax ratio in normal and lung cancer cells in low extracellular Ca2+ culture conditions. A: representative traces showing changes in [Ca2+]cyt in normal and lung cancer cells before, during, and after application of 100 μM ATP in media containing 80 nM extracellular Ca2+. B: representative traces showing changes in [Ca2+]cyt in normal and lung cancer cells before, during, and after application of 1 mM ATP in media containing 80 nM extracellular Ca2+. C: Western blot analysis of Bcl-2 and Bax expression in normal and lung cancer cells treated with 100 μM ATP in media containing 80 nM extracellular Ca2+ for 0, 2, 4, 8, 16, and 24 h. β-Actin was used as a control. D: Western blot analysis of Bcl-2 and Bax expression in normal and lung cancer cells treated with 1 mM ATP in media containing 80 nM extracellular Ca2+ for 0, 2, 4, 8, 16, and 24 h. β-Actin was used as a control. E: summarized data (means ± SE) showing the changes of Bcl-2 and Bax expression level in normal and lung cancer cells treated with 100 μM ATP in media containing 80 nM extracellular Ca2+ for 0, 2, 4, 8, 16, and 24 h. F: summarized data (means ± SE) showing the changes of Bcl-2/Bax ratio in normal and lung cancer treated with 100 μM ATP in media containing 80 nM extracellular Ca2+ for 0, 2, 4, 8, 16, and 24 h (n = 3). G: summarized data (means ± SE) showing the changes of Bcl-2 and Bax expression level in normal and lung cancer cells treated with 1 mM ATP in media containing 80 nM extracellular Ca2+ for 0, 2, 4, 8, 16, and 24 h. H: summarized data (means ± SE) showing the changes of Bcl-2/Bax ratio in normal and lung cancer cells treated with 1 mM ATP in media containing 80 nM extracellular Ca2+ for 0, 2, 4, 8, 16, and 24 h (n = 3).