Fig. 1.

Demonstration of functional Ca²⁺ transport in mDCT15 cells. A: representative Western blots demonstrating expression of plasma membrane Ca²⁺-ATPase (PMCA)1, Na⁺/Ca²⁺ exchanger (NCX)1, calbindin (Calb), transient receptor potential vanilliod (TRPV)5, and parathyroid hormone (PTH) receptor (PTH-R). B: ⁴⁵Ca²⁺ uptake in mDCT15 cells under Ca²⁺-free conditions and with Ca²⁺ and Ca²⁺ and 200 nM ruthenium red (RR sens; n = 4). *P < 0.01 compared with Ca²⁺-free conditions. Solid circles indicate means, shaded circles indicate individual data points, and bars represent SEs.