Fig. 2.
Functional properties of TRPV5-like channel in mDCT15 cells. A: representative current traces of single channel activity recorded from the same outside-out patch at different pipette potentials as indicated. c denotes the closed nonconductant state. B: average current-voltage (i-V) relationship of the unitary current amplitude for channels similar to that shown in A. Each value presented as the mean ± SE from an average of 4 individual experiments. The single channel conductance at negative voltages (27.3 ± 0.6 pS) was estimated as a slope of the linear fit. C: summary graph of channel permeability calculated as single channel amplitude multiplied by the value of open probability (Po) at any given voltage. D: representative continuous current trace from an outside-out patch monitoring activity of TRPV5-like channel in the control, upon application of 500 nM RuR (shown as the shaded bar at the top), and after washout with control media. The patch was clamped to −40 mV.