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. 2015 Nov 25;310(2):F144–F151. doi: 10.1152/ajprenal.00323.2015

Fig. 4.

Fig. 4.

Role of Ras guanyl-releasing protein (RasGRP1) on PTH stimulation of TRPV5 activity. A: Na+-Cl− cotransporter (NCC) activity with administration of 100 nM PTH for 15 min in control cells or in cells with 70% reduced RasGRP1 expression (RG KD; n = 4). *P < 0.01 compared with PTH. Solid circles indicate means, shaded circles indicate individual data points, and bars represent SEs. Inset, representative blot of RasGRP1 expression in RG KD cells. B: representative blot showing NCC surface and total expression for the above groups (n = 4). C: TRPV5 activity with administration of 100 nM PTH for 15 min in control cells and mDCT15 cells with reduced RasGRP1 expression (R). Cells were treated with 100 nM PTH (P) for 15 min with and without inhibitors of PKC (C) and PKA (A). n = 4. *P < 0.01 compared with R; #P < 0.01 compared with P; @P < 0.01 compared with R + P; &P < 0.01 compared with R + C + P. Solid circles indicate means, shaded circles indicate individual data points, and bars represent SE. D: representative blot showing TRPV5 surface and total expression for control, PTH-treated, RasGRP1 knockdown, and RasGRP1 knockdown cells treated with PTH (n = 4).