Expression of Toll-like receptors (TLRs) in fetal airway smooth muscle (ASM). Top: expression of TLR2, 3, 4, 7, and 9 was detected in fetal ASM by Western blot. Bottom: fetal ASM were treated with media only (control) for 24 h, and TLR2, 3, 4, 7, and 9 expression relative to GAPDH was evaluated. Adult ASM treated with media only was used as a positive control. Values are means ± SE; n = 3–4 samples.