Fig. 8.

Nuclear translocation of the p50 and p65 NF-κB subunits. In human fetal ASM (A) and human adult ASM (B), p50, p65, and TATA-box binding protein (TBP) in nuclear extracts, in addition to p50, p65, and GAPDH in cytoplasm extracts, were, respectively, measured by Western blot showing that TNF-α and poly(I:C) promoted nuclear translocation of the p50 and p65 NF-κB subunits in both human fetal and adult ASM. There was no global increase in either p50 or p65 expression in the cytoplasm fraction. Values are means ± SE; n = 4–7 samples. *Significant difference from control, P < 0.05.