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Immunofluorescence of NKX2.1 and pro-surfactant protein (SP)-C in normal rat lung. A: double immunofluorescence for pro-SP-C (green) and NKX2.1 (red) shows that not all NKX2.1+ cells are pro-SP-C+. Negative controls are goat IgG (gIgG) and mouse IgG (mIgG). 4′,6-Diamidino-2-phenylindole (DAPI, blue) is the nuclear counterstain. Scale bar = 50 μm. B: confocal z-stack images demonstrate that some NKX2.1+ cells are pro-SP-C− (arrows). Scale bar = 10 μm.
