Fig. 8.

LY-2584702 inhibition increases phosphorylation of 4E-binding protein (4E-BP1) and p70S6K. CCSP/TGF-α mice were administered Dox for 2 days to induce TGF-α expression. A: Western blot analysis of lung homogenates from mice treated with vehicle or LY-2584702 (25 or 50 mg/kg, 2 times/day) for phosphorylated 4E-BP1 at the Thr^37/46 sites (A) and p70S6K at the Thr³⁸⁹ site (B). Total p70S6K is the band on top (70 kDa). Right, quantification of ratio of the intensity of phosphorylated over total for each intermediate. Data are means ± SE, and statistical differences shown were performed using ANOVA (A) and an unpaired t-test (B).