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. 2015 Nov 5;310(2):G70–G80. doi: 10.1152/ajpgi.00236.2015

Fig. 5.

Fig. 5.

Pharmacological reduction of [Cl−]i normalizes pHi in Cftr KO crypt epithelium. A: [Cl−]i of crypt epithelium from Cftr KO enteroids under basal conditions (Basal) and after addition of 50 μM bumetanide (Bumet) for 15 min followed by 100 μM carbachol (Bumet + CCh) for 15 min. Values are means ± SE of 30 crypt epithelial cells in enteroids from 3 Cftr KO mice. a,b,cSignificantly different (by repeated-measures ANOVA with post hoc Tukey's test). B: pHi of crypt epithelium from Cftr KO enteroids measured before (Basal) and after sequential treatment with bumetanide (50 μM) for 15 min and CCh (100 μM) for 15 min. Values are means ± SE of 10 crypt epithelial cells in enteroids from 3 Cftr KO mice. *Significantly different from Basal (by unpaired t-test). C: effect of H2DIDS on pHi of crypt epithelium from Cftr KO enteroids measured before (Basal) and after sequential treatment with bumetanide (50 μM) for 15 min and CCh (100 μM) for 15 min. Enteroids were treated with 200 μM H2DIDS [in the basolateral (BL) solution] throughout the experiment. Values are means ± SE of 17 crypt epithelial cells in enteroids from 3 Cftr KO mice.