Fig. 4.

Localization of the TNF-α response region on human NHE8 gene promoter in goblet cells. HT29-MTX cells were transfected with human NHE8 promoter constructs pGL3B/671 (A) and pGL3B/32 (B). TNF-α were then applied 18 h before measuring promoter activities. The degree of inhibition is shown as the ratio of luciferase activity in TNF-α-treated cells over luciferase activity in nontreated cells. Results are means ± SE from 4 separate experiments. *P < 0.01 for control cells vs. TNF-α treated cells.