(A) Average maps (aligned to soma, white circle) of connection probability for excitatory connections in NR (left) and DE (right) animals. Connection probability is encoded according to the pseudocolor scale. White horizontal lines indicate averaged laminar borders and are 200 μm long. Traces in the center show the laminar marginal distributions (red for NR and black for DE). Traces at the bottom of the DE panel indicate the columnar marginal distributions. Note that NR and DE maps and distributions appear different.
(B) Average maps (aligned to soma, white circle) of connection strength (transferred charge) for excitatory inputs in NR (left) and DE (right) animals. Averages are only calculated for stimulation sites that evoked responses in >10% of cells in our sample. Connection strength is encoded according to the pseudocolor scale. White horizontal lines indicate averaged laminar borders and are 200 μm long. Traces in the center show the laminar marginal distributions (red for NR and black for DE). Traces at the bottom of the DE panel indicate the columnar marginal distributions. Note that NR and DE maps and distributions appear different.
(C) Distributions of area of input (left), total EPSC input amplitude (center), and total EPSC input charge (right) originating from L2/3 (top), L4 (middle), and L5/6 (bottom) of NR (red) or DE (black) animals. Significance: * denotes p < 0.05; ** denotes p < 0.01. EPSC area distributions: L2/3: p = 0.0218; L4: p = 0.000782; L5/6: p = 0.0518. Total EPSC amplitude distributions: L2/3: p = 0.04.91; L4: p = 0.00351; L5/6: p = 0.0891. Total EPSC charge distributions: L2/3: p = 0.0491; L4: p = 0.0040; L5/6: p = 0.0652. All comparisons were done with a KS test.
(D) Distributions of average EPSC amplitude (left) and average EPSC charge (right) originating from L2/3 (top), L4 (middle), and L5/6 (bottom) for cells from NR (red) or DE (black) animals. All p > 0.05. Average EPSC amplitudes distributions: L2/3: p = 0.364; L4: p = 0.869; L5/6: p = 0.551. Average EPSC charge distributions: L2/3: p = 0.910; L4: p = 0.918; L5/6 p = 0.644. All comparisons were done with a KS test.