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. 2015 Oct 13;11(1):139–148. doi: 10.1021/acschembio.5b00577

Figure 2.

Figure 2

(A) PCR stop assay. Compound 1 inhibits PCR amplification of a synthetic wild type oligonucleotide sequence capable of forming a G4 but does not inhibit amplification of the mutant sequence that cannot form a G4. (B) Exon specific qPCR assay with the CA-46 Burkitt’s lymphoma cell line. MYC Exon 1 (in red) remains under control of the G4, while transcription from exon 2 is not under control of a G4. Cells were treated with 10 μM 1 for the time indicated. The observed threshold cycle (Ct) by real time PCR, normalized to vehicle control, was measured. Error bars represent the standard deviation of three replicates.