Extended Data Fig. 5. Conservation of quaternary interactions between eIF3a and eIF3c subunits, and between eIF3c and eIF3e subunits.

a, ribbon representation of eIF3a and eIF3c subunits. b-d, Close-up views of contact regions between eIF3a and eIF3c. e, ribbon representation of eIF3 c and e subunits. f and g, Close-up views of contact regions between subunits c and e of eIF3. Red spheres represent residues at the interfaces that are conserved in eIF3 from six representative eukaryotic organisms; H. sapiens, C. elegans, A. thaliana, D. melanogaster, X. tropicalis, which are very different multicellular eukaryotic organisms, and N. crassa, a unicellular organism. These organisms all have a full complement of 13 eIF3 subunits. Orange spheres represent residues at the interfaces that are conserved only in the five multicellular eukaryotic organisms. The remaining residues that are suggested by the model and the density map to be involved in quaternary interactions are represented as ribbons in salmon color. Many of these other residues are conserved in three or four of the compared organisms, but almost all of them have conserved chemical properties.