Extended Data Fig. 8. Comparison of the mammalian eIF3 core model built from the 6 Å cryo-EM reconstruction with a model based on low-resolution cryo-EM studies.

In center, our polyalanine-level model of the mammalian eIF3 octamer core (represented in red ribbons) fitted on the atomic model proposed by Erzberger et al.¹⁸ (represented in dark gray ribbons), shown in two different orientations. The surrounding panels are close-up views of different constitutive subunits, highlighting significant structural differences between the previously proposed model¹⁸ and the model proposed in this study. Gold- and cyan-dashed ovals highlight the misassignment of several helices of the helical bundle belonging to the C-termini of subunits h and f, respectively, in the previously proposed model. Red-, green-dashed circles and black arrow highlight the absence in the previously proposed model of important structural features involved in quaternary interactions. In each panel, the remaining subunits of the eIF3 core octamer are faded out as transparent ribbons.