Figure 3.

Assessment of Equine iPSC Myogenic Differentiation

(A) Immunofluorescence staining for MyHC of parental cells and iPSCs under BMP/TGF-β blockade.

(B) Quantitation of differentiation efficiency as fraction of cells participating to nascent MyHC⁺ myotubes (fusion index; ^∗p < 0.05, one-way ANOVA with Bonferroni comparison, n = 3 independent experiments per cell type).

(C) qRT-PCR with equine-specific MYH2 primers; data are depicted as fold change versus MABs or MAB-iPSCs (AU, arbitrary units; ^∗p < 0.05, unpaired t test, n = 3 independent experiments per cell type). Both analyses revealed higher myogenic propensity in MAB-iPSCs than in MSC-iPSCs (n = 3 independent experiments per cell type).

Histograms represent average values; error bars indicate SD. Scale bars, 100 μm.