Fig. 8. Optimizing experimental conditions for detecting domains in DSPC/DOPC/Chol. The relative homogeneous background scattering Q hom = Q 0 + Q r, calculated from lipid NSLDs (Table 1) using eqn (24)–(29), is plotted vs. fraction of DSPC-d70 (to total DSPC) and the solvent fraction of D2O. A global contrast match point is observed at 34.6% D2O and 65.9% DSPC-d70 (“CM”, expanded in inset). Close to the contrast match point, Q hom is attenuated by >6 orders of magnitude relative to a fully protiated bilayer in 100% D2O solvent (“HC”).
