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. 2016 Jan 19;14:2. doi: 10.1186/s12959-016-0075-3

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© Ettelaie et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Time course of the microvesicle-associated TF activity released into the media by the cell lines. Cells (2 × 10⁵) were seeded out in 6-well plates and pre-adapted to respective serum-free medium. Microvesicle release was induced by incubation with PAR2-AP; SLIGRL; (20 μM). The released cell-derived microvesicles were isolated by ultracentrifugation at intervals up to 90 min and resuspended in Tris-saline. Microvesicle-associated TF activity was measured using the thrombin-generation assay, for each sample (n = 3)