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Proteasome defects or Mia40 overexpression do not prevent mitochondrial DNA loss in fzo1Δ cells. (A) The indicated yeast cells were fixed with ethanol and stained with DAPI. Images merged by differential interference contrast (DIC) are also shown. Scale bars, 5 μm. (B) Spot dilution assay. The indicated strains were sequentially diluted by 5-fold and spotted onto YPAD or YPAGly. Plates were incubated at 26°C for 2 days.
