FIG 4.

Proteasome defects rescue [rho⁰] cells and mitochondrial mutants other than fzo1Δ cells. (A and C) Spores of heterozygous diploids were dissected on YPAD prior to growth at 26°C for 4 days. Genotypes of each colony are shown. (B) Spores of MRPL35/mrpl35Δ cells harboring empty vector or Mia40-expressing plasmids were dissected on YPAD prior to growth at 26°C for 4 days. Genotypes of each colony are shown. (D) Wild-type and proteasome mutant cells were cultured on YPAGal containing 20 μg/ml ethidium bromide (EtBr) to deplete mitochondrial DNA. The resultant [rho⁰] cells and control (WT [rho⁺]) cells were sequentially diluted by 5-fold and spotted onto YPAD (left panels) or YPAGly (right panels). Plates were incubated at 26°C for 2 days.