FIG 9.

Overexpression of s-Mgm1 does not restore fzo1Δ growth phenotypes. (A) Spores of FZO1/fzo1Δ cells harboring an s-Mgm1 expression plasmid were dissected on YPAD prior to growth at 26°C for 4 days. Genotypes of each colony are shown. The triangle and pentagon indicate fzo1Δ cells with or without the s-Mgm1 plasmid, respectively. (B) The indicated strains were streaked on YPAD and YPAGly prior to growth at 26°C for 3 days. (C) The indicated strains were stained with rhodamine 123, and fluorescence intensities were measured by FACS analysis. Mean fluorescence intensities ± SD are shown. Asterisks indicate P < 0.01. n.s., not significant.