TABLE 2.
Plasmids used in this study
| Plasmid name | Background vector | Cloned gene | Reference | Function |
|---|---|---|---|---|
| p4339 | pCRII-TOPO | TEFp-natR-TEFt | 20 | Marker switch |
| KOB74 | p416 | mtDHFR-GFP | 21 | |
| KOB154 | pBSII | TEF-mtDHFR-mCherry-CgHIS3 | 70 | |
| pHY68 | pBluescript KS− | 3× FLAG-kanR | 18 | C-terminal Flag tag |
| pFA6a-hph | pFA6a | hph | 71 | Marker switch |
| YEplac195 | 72 | |||
| pRS316 | 73 | |||
| pYO325 | 17 | |||
| pYO326 | 17 | |||
| pT192 | YEplac195 | RPN4 | ||
| pUZ160 | pRS316 | SAL1BY | ||
| pUZ161 | pRS316 | SAL1W303 | ||
| pUZ206 | pYO326 | MIA40 | ||
| pUZ215 | YEplac195 | ERV1 | ||
| PUZ223 | PYO326 | TIM9-TIM10 | ||
| pUZ245 | pYO325 | MIA40 | ||
| pUZ247 | pYO325 | MGM1 | ||
| pUZ248 | pYO325 | s-MGM1 | ||
| pUZ300 | pYO326 | FL-MGM1-FLAG | ||
| pUZ301 | pYO326 | s-MGM1-FLAG |