FIG 6.
Generation and analyses of Keap1C288E knock-in mice. (A) Experimental scheme for isolation of thioglycolate-elicited peritoneal macrophages from wild-type and Keap1C288E/C288E mice. Representative sequencing data show the successful replacement of a cysteine (Cys) with glutamic acid (Glu) at position 288 (C288E) of Keap1C288E/C288E mice. (B to F) Peritoneal macrophages from WT and Keap1C288E/C288E mice treated with 0, 3, or 10 μM 15d-PGJ2 (B), 0, 30, or 100 μM DEM (C), 0, 3, or 10 μM 9-OA-NO2 (D), 0, 5, or 15 μM 4-HNE (E), or 0, 10, or 30 μM PGA2 (F) for 3 h were examined by Western blotting.