FIG 8.

Dissection of three major cysteine residues. (A) Schematic presentations of Keap1^C151S and Keap1^C273W&C288E structures. (B) Keap1^C151S and Keap1^C273W&C288E protein levels of stable complemented MEFs examined by Western blotting. (C to E) Keap1^WT and Keap1^C151S MEFs were treated with 0, 30, or 100 μM DEM (C), 0, 1, or 3 μM SFN (D), or 0, 10, or 30 μM tBHQ (E) for 3 h were examined by Western blotting. (F to J) Keap1^WT, Keap1^C151S, and Keap1^C273W&C288E MEFs were treated with 0, 3, or 10 μM 9-OA-NO₂ (F), 0, 5, or 15 μM 4-HNE (G), 0, 3, or 10 μM NaAsO₂ (H), 0, 100, or 300 μM SNAP (I), or 0, 10, or 30 nM CDDO-Im (J) for 3 h were examined by Western blotting.