FIG 6.

PKCδ and Bcl10 are involved in S1P-mediated NF-κB activation. (A) M2 cells were serum starved overnight and stimulated with S1P (100 nM) for the indicated times. (B and C) M2 cells were transfected with control siRNA or siRNA directed against PKCδ (B) or Bcl10 (C). After 48 h, cells were serum starved overnight and stimulated with S1P (100 nM). Cell lysate proteins were analyzed by immunoblotting with the indicated antibodies. Tubulin (A and C) or p65 (B) was used to ensure equal loading and transfer. Blots are representative of results from two independent experiments. Numbers indicate relative protein levels determined by densitometry.