FIG 6.

Sorbs2 knockdown inhibits AChR clustering in muscle cells. (A) Myotubes were transfected with Sorbs1 or a nontargeting siRNA, and total RNA was isolated 48 h later. Total Sorbs2 transcript levels were assessed by quantitative PCR and found to be modestly elevated (∼25%) in myotubes treated with Sorbs1 siRNAs. (B) Myotubes were transfected with siRNAs directed against Sorbs1, Sorbs2, or a nontargeting control (siScr). Cell lysates were probed with antibodies directed against Sorbs2. Sorbs2 protein is significantly reduced by Sorbs2 RNAi treatment and modestly increased with Sorbs1 RNAi treatment. (C) Knockdown of Sorbs2 protein levels inhibits AChR clustering independent of Sorbs1.