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. 2016 Jan 15;30(2):133–148. doi: 10.1101/gad.271288.115

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© 2016 Barrales et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Lem2 cooperates with Csi1 and Dsh1 in centromere and telomere positioning. (A) Representative pictures of two-color live-cell imaging of Mis6-GFP (centromere) and Sad1-mCherry (SPB). Dotted lines delineate cell boundaries. (B) Quantification of cells with distinct numbers of Mis6 foci. Shown is the percentage for a population of n cells of one representative experiment. (C) Quantification of cells displaying distinct degrees of colocalization of Mis6-GFP and Sad1-mCherry. For each cell, the most proximal Mis6-GFP focus next to the SPB is classified as overlapping, proximal, or completely separated from the SPB. Shown is the percentage of n cells of a representative experiment, denoting each type of localization. (D) Zone designation (I–III) and distribution of Taz1-GFP (telomere) within confocal planes with representative pictures (shown at right). The nuclear envelope is visualized by mCherry-Cut11. (E) Quantification of Taz1-GFP distribution relative to the nuclear periphery. Shown is the percentage of telomeres for each nuclear zone for a population of n cells combined from two independent experiments. The asterisks denote P < 0.05 (*) and P < 0.0001 (****) from χ² test analysis.