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. 2016 Jan 15;30(2):149–163. doi: 10.1101/gad.268797.115

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© 2016 Starnes et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — PTIP and PA1 protein stability and localization in cells. (A) A Western blot for PTIP and PA1 levels in B cells stimulated in the IgG3 condition for 3 d from individual PA1^−/−, PTIP^−/−, and control mice (PA1^−/− and PTIP^−/−, n = 3; control mice, n = 2). (B) A Western blot for PTIP and PA1 levels in immortalized PTIP^−/−, PA1^−/−, and control (+/+) MEFs transduced with FL and PA1. (C–F) Immunofluorescence of immortalized MEFs. (C) PA1^−/− cells transduced with untagged PA1 or empty vector control. (D) PTIP^−/− cells transduced with N-terminal GFP-tagged FL. (E) PTIP^−/− and controls (+/+) transduced with untagged-PA1. (F) PA1^−/− and controls (+/+) transduced with FL. MEFs in C–F were irradiated with 10 Gy, and FL (cyan, except for D, where FL is indicated in magenta), PA1 (cyan), and γH2AX (magenta) IR-induced foci were assessed 4 h after IR. DAPI is indicated in gray. Bars, 10 μm.