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. 2016 Jan 19;36(3):407–420. doi: 10.1128/MCB.00785-15

FIG 11.

FIG 11

MED16 regulates RNAP II CTD phosphorylation without affecting NRF2 binding to the ARE. (A) Positions of primer sets used for ChIP assay within each target gene locus (Nqo1, Gclm, and Gclc). TSS, transcription start site. Blue, green, and red arrows indicate the positions of primer sets for examining NRF2 binding to the ARE, S5P-CTD levels around the TSS, and S2P-CTD levels at the downstream region of each gene, respectively. Scale bars indicate 2 kbp. (B and C) Quantitative ChIP assay at each target gene locus in WT and Med16 KO Hepa1c1c7 cells. Localization of NRF2 and CBP (B) and of RNAP-CTD, S5P-CTD, and S2P-CTD (C) was examined in WT and Med16 KO cells treated with DEM or vehicle. Med16 KO 1 cells were used. Error bars show standard deviations. *, P < 0.05; **, P < 0.01; n.s., not significant.