TABLE 1.
Primers and probes used for mouse (m) and human (h) mRNA expression
| Primer or probe | Sequence (5′–3′)a |
|---|---|
| mNqo1 forward | AGCTGGAAGCTGCAGACCTG |
| mNqo1 reverse | CCTTTCAGAATGGCTGGCA |
| mNqo1 probe | FAM-ATTTCAGTTCCCATTGCAGTGGTTTGGG-TAMRA |
| mGpx2 forward | TGTCAGAACGAGGAGATCCTG |
| mGpx2 reverse | GACTAAAGGTGGGCTGGTACC |
| mGclm forward | TGACTCACAATGACCCGAAA |
| mGclm reverse | GATGCTTTCTTGAAGAGCTTCCT |
| mGclc forward | ATCTGCAAAGGCGGCAAC |
| mGclc reverse | ACTCCTCTGCAGCTGGCTC |
| mGclc probe | FAM-ACGGGTGCAGCAAGGCCCA-TAMRA |
| mGlut1 forward | CCATGGATCCCAGCAGCAAG |
| mGlut1 reverse | CCAGTGTTATAGCCGAACTGC |
| mBnip3 forward | GTTACCCACGAACCCCACTTT |
| mBnip3 reverse | GTGGACAGCAAGGCGAGAAT |
| mPgk1 forward | GATGCTTTCCGAGCCTCACTGT |
| mPgk1 reverse | ACCAGCCTTCTGTGGCAGATTC |
| mVegfa forward | CTGCTGTAACGATGAAGCCCTG |
| mVegfa reverse | GCTGTAGGAAGCTCATCTCTCC |
| m18SrRNA forward | CGGCTACCACATCCAAGGAA |
| m18SrRNA reverse | GCTGGAATTACCGCGGCT |
| m18SrRNA probe | FAM-TGCTGGCACCAGACTTGCCCTC-TAMRA |
| hNqo1 forward | GTCATTCTCTGGCCAATTCAGAGT |
| hNqo1 reverse | TTCCAGGATTTGAATTCGGG |
| hNqo1 probe | FAM-ACTGACATATAGCATTGGGCACACTCCAG-TAMRA |
| hGCLM forward | TAGAATCAAACTCTTCATCATCAACTAGA |
| hGCLM reverse | TCACAGAATCCAGCTGTGCAA |
| hGCLM probe | FAM-TGCAGTTGACATGGCCTGTTCAGTCC-TAMRA |
| hGCLC forward | TCTCTAATAAAGAGATGAGCAACATGC |
| hGCLC reverse | TTGACGATAGATAAAGAGATCTACGAA |
| hGCLC probe | FAM-CAGGAGATGATCAATGCCTTCCTGCAAC-TAMRA |
| hCA9 forward | CCTTTGCCAGAGTTGACGAG |
| hCA9 reverse | GACAGCAACTGCTCATAGGC |
| hBNIP3 forward | CCAAGAGCTCTCACTGTGAC |
| hBNIP3 reverse | GCTCTGTTGGTATCTTGTGG |
| hPGK1 forward | CTAACAAGCTGACGCTGGAC |
| hPGK1 reverse | CTGGTTGTTTGTTATCTGGTTG |
| hNRF2 forward | TCATGATGGACTTGGAGCTG |
| hNRF2 reverse | CATACTCTTTCCGTCGCTGA |
| hHPRT forward | CCGGCTCCGTTATGGC |
| hHPRT reverse | GGTCATAACCTGGTTCATCATCA |
| hHPRT probe | FAM-CGCAGCCCTGGCGTCGTGATTA-TAMRA |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.