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Sequence analysis and purification of the flagellar rod components from R. sphaeroides. (A) Amino acid sequence alignment of the N and C termini of the four rod proteins. Sixty residues of each terminal region were aligned using the program MUSCLE. Conserved residues are highlighted, and the length of each protein is listed at the end of the alignment. (B) SDS-PAGE analysis of purified N-terminal His-tagged rod component proteins, with Coomassie blue staining.
