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Effects of the N- and C-terminal regions of FlgF and FlgG on heteromeric interactions, tested by affinity blotting. (A) FlgF and FlgF49–212 (0.05 nmol) were transferred to a nitrocellulose membrane, and FlgB was used as the probe. (B) FlgG and FlgG47–227 (0.05 nmol) were transferred to a nitrocellulose membrane, and FlgC was used as the probe. Detection was performed by using specific antibodies against the probes. Molecular mass markers are shown on the right (in kilodaltons).
