TABLE 1.
Bacterial strains, plasmids, and oligonucleotides
| Strain, plasmid, or oligonucleotide | Relevant characteristic(s) or sequence | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| TOP10 | Cloning strain | Invitrogen |
| JM109 | hdsR17 Δ(lac-pro) F′ traD36 proAB lacIqZΔM15 | Novagen |
| M15[pREP4] | thi lac ara gal mtl F′ recA+ uvr+ lon+; pREP4 plasmid; Kanr | Qiagen |
| BL21(DE3)pLysS | F′ ompT hsdSB(rB− mB−) gal dcm(DE3) pLysS | Novagen |
| R. sphaeroides WS8-N | Wild type; spontaneous Nalr | 39 |
| Plasmids | ||
| pQE30 | Expression vector; Ampr; N-terminal His6 tag | Qiagen |
| pET19b | Expression vector; Ampr; N-terminal His10 tag | Novagen |
| pRSFliE | fliE cloned into SacI/HindIII sites of pQE-30 | This study |
| pRSFlgB | flgB cloned into BamHI/HindIII sites of pQE-30 | This study |
| pRSFlgC | flgC cloned into NdeI/BamHI sites of pET-19b | This study |
| pRSFlgF | flgF cloned into KpnI/HindIII sites of pQE-30 | This study |
| pRSF49 | F49 cloned into KpnI/HindIII sites of pQE-30 | This study |
| pRSFlgG | flgG cloned into NdeI/BamHI sites of pET-19b | This study |
| pRSG47 | F47 cloned into NdeI/BamHI sites of pET-19b | This study |
| Oligonucleotides | ||
| fliEfw | CGATGAGCTCATGACCATCCAGTCGATCAGC | This study |
| fliErv | CGATAAGCTTTCAGACCGGCATGTTCATGAT | This study |
| flgBfw | CGATGGATCCATGACGGGATTTCGCGATCAG | This study |
| flgBrv | CGATAAGCTTTCATTCCCCCTTGATCGCCGT | This study |
| flgCfw | GGAATTCCATATGAGCGGGATCGACAGTGTCTTC | This study |
| flgCrv | CGCGGATCCTCACTGCCCCATGCTGGCGGT | This study |
| flgFfw | CGGGGTACCGACCGGCTGATCCACACCGCG | This study |
| flgFrv | CGATAAGCTTTCACTCGGGCGGACGCAGGAG | This study |
| F49fw | CGGGGTACCGCGATGGACGCGGCCTCGGCG | This study |
| F49rv | CGATAAGCTTTCACGTATTGACGTTCGAGCCATA | This study |
| flgGfw | GGAATTCCATATGTCCACCAATGCGATGCATGTC | This study |
| flgGrv | CGCGGATCCTCAGAGCTTGTTGGAAAGATA | This study |
| G47fw | GGAATTCCATATGTACCAGACCTGGAAGCCCGGC | This study |
| G47rv | CGCGGATCCTCACACGTTGACGTTCGAGGCTTC | This study |