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A TcpH-C114S mutant cannot stabilize wild-type TcpP. TcpP and TcpH were cotranscribed from the pBAD18-based plasmid pGOOD-tcpPH encoding either wild-type TcpH or TcpH-C114S. Strains were induced for tcpPH expression with 0.1% arabinose, and levels of TcpP (A) and TcpH (B) were determined by Western blotting.
