TABLE 2.

Glycine improves growth rates of ridA strains inhibited by 2AA

Growth mediumb	Growth ratea (h−1) of:
	DM14828 (Wt)	DM14829 (ridA)	DM14838 (gcvP)	DM14839 (ridA gcvP)	DM14836 (gcvB)	DM14837 (ridA gcvB)
Min-glucose	0.56	0.42	0.52	0.33	0.51	0.42
+Gly	0.52	0.52	0.38	0.23	0.59	0.51
+Ser	0.57	NGc	0.59	0.28d	0.59	NG
+Ser and Gly	0.60	0.34	0.58	0.18	0.59	NG
+Cys	0.50	NG	0.54	NG	0.57	NG
+Cys and Gly	0.57	0.30	0.52	NG	0.54	0.31
+Dap	0.58	NG	0.52	NG	0.56	NG
+Dap and Gly	0.57	0.43	0.45	NG	0.49	0.51
Min-pyruvate	0.41	NG	0.40	NG	0.44	NG
+Gly	0.42	0.33	0.35	NG	0.41	0.35

^aSpecific growth rates (μ) were calculated as ln(X/X₀)/T, where X represents OD₆₅₀, X₀ is the initial OD₆₅₀ of the exponential growth period monitored, and T is time in hours. Data are the averages for three independent cultures. The standard deviation was less than 0.05 in all cases. Growth was assessed within 20 h following inoculation. Wt, wild type.

^bGlucose (11 mM) and pyruvate (50 mM) were provided as the carbon source in minimal media as indicated (Min-glucose and Min-pyruvate, respectively). Amino acid supplements were added at the following final concentrations: 0.67 mM glycine (Gly), 5 mM serine (Ser), 0.25 mM cysteine (Cys), and 0.1 mM diaminopropionate (Dap).

^cAverage growth rates less than 0.05 h⁻¹ were designated as no growth (NG).

^dThe displayed growth rate reflects the secondary growth phase of a biphasic growth pattern; the primary growth rate was 0.06 h⁻¹.