FIG 5.

Growth defect of L. monocytogenes cdaR mutant strains. (A) Growth of an L. monocytogenes cdaR mutant strain in BHI broth at 37°C. Strains EGD-e (wild type) and LMJR45 (ΔcdaR) were grown in BHI broth at 37°C and the optical densities (OD₆₀₀) were measured in hourly intervals. (B) Growth of the same set of strains in BHI broth at 42°C. (C) Complementation of the ΔcdaR growth defect at 42°C. Strains EGD-e (wild type), LMJR45 (ΔcdaR), and BPL16 (IcdaR) were cultivated in BHI broth with or without 1 mM IPTG at 42°C, and the OD₆₀₀ values were recorded over time. Average values were calculated from three independent experiments, and standard deviations are shown.