FIG 6.

Multiple-sequence alignment of SepM and its homologs. The alignment was performed using Clustal W. The degrees of shading were done using BoxShade, in which black and gray blocks indicate identical and similar amino acids, respectively. Sequences were obtained from GenBank. The organisms are S. mutans (SMU [SepM]; GenBank accession no. NP_720956), S. pyogenes (GAS; GenBank accession no. WP_010922499.1), S. agalactiae (GBS; GenBank accession no. WP_000790649.1), S. gordonii (SGO; GenBank accession no. ABV09441.1), and S. uberis (SUB; GenBank accession no. WP_015911639.1). Asterisks indicates the residues that were targeted for mutagenesis.