TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| S. mutans UA159 | Wild type | 37 |
| S. mutans IBS-D29 | Markerless deletion of SMU.518 (ΔsepM) | 8 |
| S. mutans IBS-N42 | Markerless deletion of comC (ΔcomC) | This study |
| S. mutans IBS-N43 | Markerless deletion of comC and sepM (ΔcomC ΔsepM) | This study |
| S. agalactiae NEM316 | Wild type | 38 |
| S. gordonii DL-1 | Wild type | 39 |
| S. pneumoniae ATCC 49619 | Wild type | ATCC |
| S. pyogenes AM3 | Wild type | 40 |
| S. ratti FA-1 | Wild type | ATCC |
| S. uberis UT387 | Wild type | 41 |
| Plasmids | ||
| pIB184-Km | E. coli-streptococcal shuttle plasmid | 21 |
| pIB-N1 | pIB184-Km with S. gordonii SepM | This study |
| pIB-N2 | pIB184-Km with S. pyogenes SepM | This study |
| pIB-N3 | pIB184-Km with S. agalactiae SepM | This study |
| pIB-N4 | pIB184-Km with S. ratti SepM | This study |
| pIB-N5 | pIB184-Km with SepM | This study |
| pIB-N6 | pIB184-Km with S. pneumoniae SepM | This study |
| pIB-N7 | pIB184-Km with S. uberis SepM | This study |
| pIB-N40 | pIB184-Km::SepM with an Asn141Asp substitution | This study |
| pIB-N41 | pIB184-Km::SepM with a Gln315Asp substitution | This study |
| pIB-N42 | pIB184-Km::SepM with a Gln315Glu substitution | This study |
| pIB-N43 | pIB184-Km::SGO with an Asp141Asn substitution | This study |
| pIB-N44 | pIB184-Km::SGO with a Glu315Gln substitution | This study |
| pIB-N35 | pGhost9 TR::PnlmA-gusA reporter | This study |
| pIB-D57 | pIB184Km::comC (18 residues) | 8 |
| pIB-D58 | pIB184Km::comC (21 residues) | 8 |
| pIB-D55 | pGEM-T-Easy::comC region | 8 |
| pIB-N36 | pIB-D55::ΔcomC with the loxP-Km cassette | This study |
| pIB-N11 | pIB184Km::comC with an Ala18Ala substitution | This study |
| pIB-N12 | pIB184Km::comC with an Ala18Asp substitution | This study |
| pIB-N13 | pIB184Km::comC with an Ala18Leu substitution | This study |
| pIB-N14 | pIB184Km::comC with an Ala18Gly substitution | This study |
| pIB-N15 | pIB184Km::comC with an Ala18Pro substitution | This study |
| pIB-N16 | pIB184Km::comC with an Ala18Phe substitution | This study |
| pIB-N17 | pIB184Km::comC with an Ala18Thr substitution | This study |
| pIB-N18 | pIB184Km::comC with an Ala18Val substitution | This study |
| pIB-N19 | pIB184Km::comC with an Ala18Cys substitution | This study |
| pIB-N20 | pIB184Km::comC with an Ala18Arg substitution | This study |
| pIB-N21 | pIB184Km::comC with an Ala18His substitution | This study |
| pIB-N22 | pIB184Km::comC with an Ala18Tyr substitution | This study |
| pIB-N23 | pIB184Km::comC with an Ala18Ser substitution | This study |
| pIB-N24 | pIB184Km::comC with an Ala18Glu substitution | This study |
| pIB-N25 | pIB184Km::comC with an Ala18Ile substitution | This study |
| pIB-N26 | pIB184Km::comC with an Ala18Trp substitution | This study |
| pIB-N27 | pIB184Km::comC with an Ala18Gln substitution | This study |
| pIB-N28 | pIB184Km::comC with an Ala18Lys substitution | This study |
| pIB-N29 | pIB184Km::comC with an Ala18Asn substitution | This study |
| pIB-N30 | pIB184Km::comC with an Ala18Met substitution | This study |
| pIB-N37 | pIB184Km::comC with a Leu18Ile substitution | This study |
| pIB-N38 | pIB184Km::comC with a Leu18Ala substitution | This study |
| pIB-N39 | pIB184Km::comC with a Leu18Val substitution | This study |
a
SGO, S. gordonii SepM.