TABLE 2.
List of oligonucleotides used in this study
| Name | Sequence (5′ to 3′) | Purposea |
|---|---|---|
| BamH-SepM-F | CGGGATCCATGCCCGGCGGAGCCTAT | Purification of SepM |
| Pst-SepM-R | AAAACTGCAGAGTGTTTTCTTAGGTAA | Purification of SepM |
| BamHI SMU.518-F | GCGGGATCCTTGGGGCAGACATCAGTCCTT | Cloning of SepM in pIB184 |
| XhoI SMU.518-R | GCGCTCGAGTTAGTGTTTTCTTAGGTAATC | Cloning of SepM in pIB184 |
| BamHI GBS0502-F | GCGGGATCCGTTCCAAAGAGTGTTGTTAGA | Cloning of GBS homolog in pIB184 |
| XhoI GBS0502-R | GCGCTCGAGCCGAACTCTTTGAGTTTATTT | Cloning of GBS homolog in pIB184 |
| BamHI SGO_0652-F | GCGGGATCCTATGTGCCCCAAAGTGTAATC | Cloning of SGO homolog in pIB184 |
| XhoI SGO_0652-R | GCGCTCGAGGCCTCAGCTTTATTATTTATT | Cloning of SGO homolog in pIB184 |
| BamHI Spy1536-F | GCGGGATCCTAGAAGGTTTGGTTCCTCAGT | Cloning of GAS homolog in pIB184 |
| XhoI Spy1536-R | GCGCTCGAGTTATTTGCGAAGATAAACCAG | Cloning of GAS homolog in pIB184 |
| SMU.518 a421g-F | TTTATGTTTTGCAGGTTAGTAGGGATTCAACATTCAAGGGTGTTTTA | Mutagenesis of SepM (N141D) |
| SMU.518 a421g-R | TAAAACACCCTTGAATGTTGAATCCCTACTAACCTGCAAAACATAAA | Mutagenesis of SepM (N141D) |
| SMU.518 c943g-F | CCCAAAGCTCTCACTAATTATGAAGAAGCTAAGCAGGC | Mutagenesis of SepM (Q315E) |
| SMU.518 c943g-R | GCCTGCTTAGCTTCTTCATAATTAGTGAGAGCTTTGGG | Mutagenesis of SepM (Q315E) |
| SMU.518 c943g_a945c-F | AAAAAAATCCCAAAGCTCTCACTAATTATGACGAAGCTAAGCAGGCTG | Mutagenesis of SepM (Q315D) |
| SMU.518 c943g_a945c-R | CAGCCTGCTTAGCTTCGTCATAATTAGTGAGAGCTTTGGGATTTTTTT | Mutagenesis of SepM (Q315D) |
| SGO g421a-F | GTCTATGTTTTGCAAGTCGCAAAAAATTCGACTTTTAAGGGAATTTT | Mutagenesis of SGO (D141N) |
| SGO g421a-R | AAAATTCCCTTAAAAGTCGAATTTTTTGCGACTTGCAAAACATAGAC | Mutagenesis of SGO (D141N) |
| SGO g943c-F | CGATCCAAAAGCAAAGACAAACTATCAAACTGCAGTTGAG | Mutagenesis of SGO (E315Q) |
| SGO g943c-R | CTCAACTGCAGTTTGATAGTTTGTCTTTGCTTTTGGATCG | Mutagenesis of SGO (E315Q) |
| comC g127 c128-F | GGCTGTTTAACAGAAGTTTTACACAANNTTTGGGAAAATAACTCGAGATCTA | Mutagenesis of CSP-21 (A18) |
| comC g127 c128-R | TAGATCTCGAGTTATTTTCCCAAANNTTGTGTAAAACTTCTGTTAAACAGCC | Mutagenesis of CSP-21 (A18) |
| comC c128 t129-F | CTGTTTAACAGAAGTTTTACACAAGNNTTGGGAAAATAACTCGAGATCTATC | Mutagenesis of CSP-21 (A18) |
| comC c128 t129-R | GATAGATCTCGAGTTATTTTCCCAANNCTTGTGTAAAACTTCTGTTAAACAG | Mutagenesis of CSP-21 (A18) |
| comC g127a_c128t-F | GGCTGTTTAACAGAAGTTTTACACAAATTTTGGGAAAATAACTCGAGATCTA | Mutagenesis of CSP-21 (A18) |
| comC g127a_c128t-R | TAGATCTCGAGTTATTTTCCCAAAATTTGTGTAAAACTTCTGTTAAACAGCC | Mutagenesis of CSP-21 (A18) |
| comC g127t_c128g_t129g-F | TTTTTCCGGCTGTTTAACAGAAGTTTTACACAATGGTTGGGAAAATAACTCGAGATC | Mutagenesis of CSP-21 (A18) |
| comC g127t_c128g_t129g-R | GATCTCGAGTTATTTTCCCAACCATTGTGTAAAACTTCTGTTAAACAGCCGGAAAAA | Mutagenesis of CSP-21 (A18) |
| comC g127c_c128a_t129g-F | TTTTTCCGGCTGTTTAACAGAAGTTTTACACAACAGTTGGGAAAATAACTCGAGATC | Mutagenesis of CSP-21 (A18) |
| comC g127c_c128a_t129g-R | GATCTCGAGTTATTTTCCCAACTGTTGTGTAAAACTTCTGTTAAACAGCCGGAAAAA | Mutagenesis of CSP-21 (A18) |
| comC g127a_c128a_t129s-F | TTTTTCCGGCTGTTTAACAGAAGTTTTACACAAAASTTGGGAAAATAACTCGAGATC | Mutagenesis of CSP-21 (A18) |
| comC g127a_c128a_t129s-R | GATCTCGAGTTATTTTCCCAASTTTTGTGTAAAACTTCTGTTAAACAGCCGGAAAAA | Mutagenesis of CSP-21 (A18) |
| comC g127a_c128t_t129g-F | TTTTTCCGGCTGTTTAACAGAAGTTTTACACAAATGTTGGGAAAATAACTCGAGATC | Mutagenesis of CSP-21 (A18) |
| comC g127a_c128t_t129g-R | GATCTCGAGTTATTTTCCCAACATTTGTGTAAAACTTCTGTTAAACAGCCGGAAAAA | Mutagenesis of CSP-21 (A18) |
| comC t211g_t212c-F | GGCTGTTTAACAGAAGTTTTACACAAGCTGCGGGAAAATAACTCGAG | Mutagenesis of CSP-21 L19A |
| comC t211g_t212c-R | CTCGAGTTATTTTCCCGCAGCTTGTGTAAAACTTCTGTTAAACAGCC | Mutagenesis of CSP-21 L19A |
| comC t211g-F | GGCTGTTTAACAGAAGTTTTACACAAGCTGTGGGAAAATAACTCG | Mutagenesis of CSP-21 L19V |
| comC t211g-R | CGAGTTATTTTCCCACAGCTTGTGTAAAACTTCTGTTAAACAGCC | Mutagenesis of CSP-21 L19V |
| comC t211a_g213c-F | CGGCTGTTTAACAGAAGTTTTACACAAGCTATCGGAAAATAACTCGAGAT | Mutagenesis of CSP-21 L19I |
| comC t211a_g213c-R | ATCTCGAGTTATTTTCCGATAGCTTGTGTAAAACTTCTGTTAAACAGCCG | Mutagenesis of CSP-21 L19I |
| comC 5′-F | GTCTTTTTTACATATTTTATTCCCC | Deletion of comC |
| comC 3′-R | TTGATTCCAATGAATATTACTATGC | Deletion of comC |
| KpnI pnlmA-gusA-F | GGGGTACCTACAAATATGGCAATCGAAGTTTTGG | Amplification of PnlmA-gusA |
| PstI pnlmA-gusA-R | AACTGCAGTTGTTTGCCTCCCTGCTGCGGTTTT | Amplification of PnlmA-gusA |
| lox77-Kan-F | CGTACCGTTCGTATAGCATACATTATACGAAGTTATGAGGATGAAGAGGATGAGGAGGCAG | Amplification of lox-Km cassette |
| lox66-Kan-R | CGTACCGTTCGTATAATGTATGCTATACGAAGTTATGCTTTTTAGACATCTAAATCTAGG | Amplification of lox-Km cassette |
a
SGO, S. gordonii SepM.