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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Dec 13;469(3):606–612. doi: 10.1016/j.bbrc.2015.11.130

Figure 3. ST3Gal-I reaction mechanism.

Figure 3

A. Michaelis-Menten plot obtained to study bi-bi mechanism by varying CMP-Neu5Ac from 0.008–0.5mM in the presence of 1mM and 0.5mM [3]/[4], respectively. B. ST3Gal-I reaction kinetics in the presence of 1mM [3] (Substrate B), and different fixed concentrations of CMP (Substrate Q). C. Experiments identical to panel B were performed only upon varying [3] in the presence of fixed 1mM CMP-Neu5Ac (Substrate A). All reactions were carried out in the presence of 1 mU/ml (panel A) and 0.1mU/ml (panel B, C) ST3Gal-I, respectively.