FIG 5.

Pharmacologic inhibition of Hsc70 represses MHV68 replication. (A) 3T3 fibroblasts were treated with vehicle (DMSO) or the indicated concentrations of Hsc70 inhibitor VER-155008 for 1 h prior to and during infection with MHV68 at an MOI of 5 PFU/cell. Cells were harvested at the indicated times postinfection, and viral titers were determined by plaque assay. Results are means for triplicate samples. Error bars represent standard deviations. (B) 3T3 fibroblasts were mock infected or infected with MHV68 in the presence of PAA (200 μg/ml) to inhibit viral replication-related cell death and the indicated concentrations of VER-155008. A separate group of cells were treated with cycloheximide (10 ng/ml) and tumor necrosis factor alpha (TNF-α) (0.2 μg/ml) as a positive control for cell death. Cell viability was determined at 48 h posttreatment by crystal violet retention assays. Data represent percentages of cell death relative to mock-treated controls. Results are means for triplicate samples. Error bars represent standard deviations.