FIG 9.

The effect of Hsc70 inhibition on viral replication is less severe for mLANA-null MHV68. 3T3 fibroblasts were treated with vehicle (DMSO) or the indicated concentrations of Hsc70 inhibitor VER-155008 for 1 h prior to and during infection with either WT MHV68 (A) or 73.STOP virus (B) at an MOI of 5 PFU/cell. Cells were harvested at the indicated times postinfection, and viral titers were determined by plaque assay. Results are expressed as the mean viral yield of triplicate samples (viral titers at 24 or 48 h divided by mean viral titers for 0 h) for that particular time point. Error bars represent standard deviations. Statistical significance was determined by performing Student's t test, with a 95% confidence interval comparing the indicated treatments. *, P < 0.05; **, P < 0.01; n.s., not significant (P > 0.05).