TABLE 1.

Conserved mLANA interactions scored by I-DIRT

Gene symbol	Gene name	Molecular mass (kDa)	Assigned spectraa	KSHV LANA interaction reference(s)	L/L+Hb	Specificityc
HSP7C	Heat shock cognate 71-kDa protein	71	30	71	0.7828	Specific
H12	Histone H1.2	21	23	8	0.7743	Specific
EF1A1	Elongation factor 1 alpha	50	24	40	0.7693	Specific
ACTB	Actin, cytoplasmic 1	42	174	39, 40	0.7582	Specific
RL12	Rpl12 60S ribosomal protein L12	18	16	40	0.7319	Nonspecific
NPM	Nucleophosmin	33	25	40, 41	0.7286	Nonspecific
RS4X	40S ribosomal protein S4	30	27	39	0.7282	Nonspecific
ANXA2	Annexin A2	39	19	40	0.7110	Nonspecific
RS5	40S ribosomal protein S5	20	17	40	0.7027	Nonspecific
TBB5	Tubulin beta-5 chain	50	46	39	0.7008	Nonspecific
RS18	40S ribosomal protein S18	18	10	40, 41	0.6995	Nonspecific
RL11	60S ribosomal protein L11	20	13	40	0.6964	Nonspecific
HNRPK	Heterogeneous nuclear ribonucleoprotein K	51	6	40, 42	0.6722	Nonspecific
HS90B	Heat shock protein 90	83	20	40, 71	0.6685	Nonspecific
ANXA1	Annexin A1	39	9	40	0.6564	Nonspecific
KPYM	Pyruvate kinase isozyme M2	58	8	40	0.6550	Nonspecific
H2AJ	Histone H2A.J	14	14	41, 64	0.6189	Nonspecific
HNRPM	Heterogeneous nuclear ribonucleoprotein M	78	25	41	0.6049	Nonspecific
MYH9	Myosin 9	226	8	39	0.5868	Nonspecific

^aNumber of tryptic peptides detected for that particular protein.

^bL/L+H is the fraction of light isotopic peptides, which is defined as the light isotopic peptide abundance divided by a light and heavy isotopic peptide mixture for any given protein.

^c“Specific” interactions are defined as those interactions which possess an L/L+H value of >0.7528 or one standard deviation above the mean light fraction threshold of 21 ribosomal proteins, which equals 0.6918. Interactions with an L/L+H value of <0.7528 were considered “nonspecific.”