FIG 6.

Determination of the region responsible for ERV-DC7 fl dysfunction. (A) Schematic representation of expression constructs coding recombinant Env composed of ERV-DC7 fl and ERV-DC14 Env. The names of constructs indicate the range of amino acid sequences from ERV-DC7 fl (the only exception is DC7/r401-472, whose name indicates the range from ERV-DC14 Env). Sp, signal peptide; SU, surface subunit; TM, transmembrane subunit; N-term, SU N-terminal domain; PRR, proline-rich region; C-term, SU C-terminal domain. (B) Immunoblotting analysis, in which Env proteins were expressed in GP Lac cells and detected in the cell lysates with anti-FeLV SU antibody. (C) Infection assay using Env-pseudotyped viruses. Fresh HEK293T cells were inoculated with pseudotyped viruses of chimeric Env, and after 48 h, X-Gal-positive cells were counted as infectious units (I.U.).