TABLE 1.
Expression plasmids constructed by site-directed mutagenesis in this study
| Expression plasmid | Template plasmid | Forward primer | Reverse primer |
|---|---|---|---|
| pFUΔss ERV-DC7 fl | pFUΔss ERV-DC7 env | Fe-246S | Fe-218R |
| Fe-247S | Fe-219R | ||
| pFUΔss ERV-DC16 fl | pFUΔss ERV-DC16 env | Fe-248S | Fe-220R |
| Fe-249S | Fe-221R | ||
| pFUΔss DC14/G407R | pFUΔss DC14 | Fe-344S | Fe-281R |
| pFUΔss DC14/N427I | pFUΔss DC14 | Fe-345S | Fe-282R |
| pFUΔss DC14/N427T | pFUΔss DC14 | Fe-378S | Fe-411R |
| pFUΔss DC14/A429T | pFUΔss DC14 | Fe-346S | Fe-283R |
| pFUΔss DC14/Y431D | pFUΔss DC14 | Fe-358S | Fe-395R |
| pFUΔss DC14/G457S | pFUΔss DC14 | Fe-347S | Fe-284R |
| pFUΔss DC14/N427I-A429T | pFUΔss DC14 | Fe-382S | Fe-413R |
| pFUΔss DC7 fl/R407G | pFUΔss ERV-DC7 fl | Fe-362S | Fe-401R |
| pFUΔss DC7 fl/ | |||
| R407G-I427N-T429A | pFUΔss DC7/r401-472 | Fe-376S | Fe-409R |
| pFUΔss DC16 fl/D431Y | pFUΔss ERV-DC16 fl | Fe-363S | Fe-402R |
| pFUΔss Fr-MLV/407R | pFUΔss Fr-MLV Env | Fr-MLV mu1 S | Fr-MLV mu1 R |
| pFUΔss Fr-MLV/431D | pFUΔss Fr-MLV Env | Fr-MLV mu5 S | Fr-MLV mu5 R |
| pFUΔss FeLV-A/407R | pFUΔss A5 | Fe-379S | Fe-412R |
| pFUΔss FeLV-A/431D | pFUΔss A5 | Fe-380S | Fe-413R |
| pFUΔss ERV-DC10/407R | pFUΔss DC10 | Fe-344S | Fe-281R |
| pFUΔss ERV-DC10/431D | pFUΔss DC10 | Fe-377S | Fe-410R |
| pFUΔss KoRV-A/407R | pFUΔss KoRV-A_1 Env | KoRV mu1 S | KoRV mu1 R |
| pFUΔss KoRV-A/431D | pFUΔss KoRV-A_1 Env | KoRV mu5 S | KoRV mu5 R |
| pFUΔss KoRV-A-Myc/407R | pFUΔss KoRV-A-Myc | KoRV mu1 S | KoRV mu1 R |
| pFUΔss KoRV-A-Myc/431D | pFUΔss KoRV-A-Myc | KoRV mu5 S | KoRV mu5 R |
Primer sequences are shown in Table S1 in the supplemental material.