TABLE 2.

Expression plasmids constructed by the seamless cloning method in this study

Expression plasmid	Template plasmid	Forward primer	Reverse primer
pFUΔss DC7/-300	pFUΔss ERV-DC7 fl	Fe-327S	Fe-267R
	pFUΔss DC14	Fe-328S	Fe-266R
pFUΔss DC7/-400	pFUΔss ERV-DC7 fl	Fe-327S	Fe-268R
	pFUΔss DC14	Fe-329S	Fe-266R
pFUΔss DC7/-472	pFUΔss ERV-DC7 fl	Fe-327S	Fe-269R
	pFUΔss DC14	Fe-330S	Fe-266R
pFUΔss DC7/401-472	pFUΔss DC14	Fe-327S	Fe-268R
	pFUΔss DC7/-472	Fe-329S	Fe-266R
pFUΔss DC7/r401-472	pFUΔss DC7/-400	Fe-327S	Fe-269R
	pFUΔss ERV-DC7 fl	Fe-330S	Fe-266R
pFUΔss DC16/-300	pFUΔss ERV-DC16 fl	Fe-327S	Fe-267R
	pFUΔss DC14	Fe-328S	Fe-266R
pFUΔss DC16/-400	pFUΔss ERV-DC16 fl	Fe-327S	Fe-268R
	pFUΔss DC14	Fe-329S	Fe-266R
pFUΔss DC16/-472	pFUΔss ERV-DC16 fl	Fe-327S	Fe-269R
	pFUΔss DC14	Fe-330S	Fe-266R
pFUΔss DC16/401-472	pFUΔss DC14	Fe-327S	Fe-268R
	pFUΔss DC16/-472	Fe-329S	Fe-266R

Primer sequences are shown in Table S1 in the supplemental material.