TABLE 3.
Viral interference assay using LacZ-carrying Env pseudotyped viruses
| Env pseudotype | Titer (IU/ml) in HEK293T cells preinfected with: |
||||
|---|---|---|---|---|---|
| No virus | FeLV-A | FeLV-B | FeLV-D | ERV-DC10 | |
| Mock | <1 | <1 | <1 | <1 | <1 |
| ERV-DC14 Env | 7.3 × 103 | 5.8 × 103 | 8.1 × 103 | <1 | 7.2 × 103 |
| ERV-DC7 rec | 3.1 × 103 | 2.7 × 103 | 3.0 × 103 | <1 | 3.1 × 103 |
| ERV-DC16 rec | 2.3 × 103 | 3.5 × 103 | 3.9 × 103 | <1 | 2.7 × 103 |
| FeLV-A/Glasgow-1 | 4.1 × 103 | <1 | 3.8 × 103 | 3.2 × 103 | 2.6 × 103 |
| FeLV-B/Gardner-Arnstein | 1.2 × 104 | 1.2 × 104 | <1 | 1.7 × 104 | 1.5 × 104 |
| FeLV-D/ON-T | 7.8 × 103 | 6.1 × 103 | 9.4 × 103 | <1 | 7.4 × 103 |
| ERV-DC10 | 3.7 × 103 | 2.6 × 103 | 2.1 × 103 | 2.8 × 103 | <1 |
| Amphotropic MLV/4070A | 2.1 × 104 | 1.8 × 104 | 2.2 × 104 | 1.9 × 104 | 1.8 × 104 |
HEK293T cells were inoculated with Env-pseudotyped viruses. Mock, empty plasmid. X-Gal-positive cells were counted as infectious units (IU) at 48 h postinfection.