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Effect of P1 deoptimization on specific infectivity
| Virus and treatmenta | Titer (PFU/ml) | VP/mlb | Specific infectivity (VP/PFU) |
|---|---|---|---|
| A12-WT | |||
| PEG ppt | 2.3 × 109 | ||
| SDG | 8.5 × 108 | 4.42 × 1012 | 5,200 |
| A12-P1 deopt | |||
| PEG ppt | 4.4 × 108 | ||
| SDG | 3.4 × 108 | 9.4 × 1012 | 27,631 |
PEG ppt, concentration by polyethylene glycol precipitation; SDG, purification by sucrose gradient centrifugation.
Viral particles per milliliter, calculated by reading the optical density at 260 nm (E1% = 76).
