FIG 6.

Group I PAKs are involved in US3-mediated modulation of CD300a binding and PS exposure. SK cells were either mock infected or infected with WT or US3-null PRV (NIA3 strain). At 2 hpi, the group I PAK inhibitor IPA-3 (10 μM) or DMSO (as a diluent control) was added. (A) At 12 hpi, cells were assessed by flow cytometry for the binding of recombinant CD300a-Fc (1 μg/sample) (left) and for PS exposure on the cell surface (by using antibody 1H6) (right). Graphs show means + SEM for three independent repeats (***, P < 0.001). (B) At 12 hpi, cells were assessed by Western blotting for the expression of PRV gB, PRV gD, PRV US3, and tubulin.