FIG 2.

Characterization of the generated avian-mammalian reassortant RVA and its parent viruses, SA11 and 02V0002G3. Cell culture supernatants were analyzed 4 days after infection of MA-104 cells. (A) RT-PCR analysis using segment- and strain-specific primers (listed in Table S1 in the supplemental material), which generate products of approximately 400 bp in the case of strain SA11 and those of approximately 200 bp in the case of strain 02V0002G3. (B) Negative-stain electron microscopy, uranyl acetate staining.